Innovative Software to maximise LC(MS) productivity for physico-chemical measurements

A pharmaceutical physical chemistry group will typically run a variety of assays such as chemical stability, solubility, lipophilicity measurement, PAMPA and binding assays. With the high cost of ownership of LC(MS) systems there is always a need to maximise the return on investment in these systems by running as many different assays on the minimum number of systems to take full advantage of their inherent speed and versatility.

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Validation of a PAMPA permeability assay

Over recent weeks I have been working to establish a suite of assays using my own UHPLC instrumentation.  LogD, solubility and PAMPA permeability measurement are all now available to clients via out-sourcing, in-sourcing or consultancy to set up the methods in your laboratory which can also include bespoke software. Here I am presenting some validation data to support the use of my PAMPA method.

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Validation of a Chromatographic method for LogD measurement

This blog returns to the familiar theme of physico-chemical measurement.  Over recent weeks I have been working to establish a suite of assays using my own UHPLC instrumentation.  LogD, solubility and PAMPA permeability measurement are all now available to clients via out-sourcing, in-sourcing or consultancy to set up the methods in your laboratory which can also include bespoke software. Here I am presenting some validation data to support the use of my LogD7.4 method.

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Antibody analysis using High Performance Capillary Electrophoresis (HPCE)

This post features some data generated by deltaDOT Ltd using HPCE for the analysis of the monoclonal antibodies Infliximab and Rituximab. Stability, fragmentation and charge heterogeneity were assessed using a combination of Capillary Gel Electrophoresis (CGE) and Capillary Zone Electrophoresis (CZE). Quantifiable differences were observed in the samples before and after heat-stress. Excellent repeatability was obtained. This work was performed as part of a study for the UK’s National Health Service (NHS).

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What discovery companies need from external partners

The rise in the importance of out-sourcing has been one of the most dramatic changes in the drug discovery landscape over the past 20 years. SMEs requiring to out-source some or all of their activities need flexible, responsive partners focussed on providing them with the clear solutions they need to move their programmes forward.

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Discovery Formulation

In this article I have discussed several aspects of Formulation in a Drug Discovery setting. This includes screening, stability and solubility for solution and suspension formulations. The related area of analysis of dosing solutions and suspensions to support both PK and PD studies is also covered. For suspension formulations, it is possible to generate information relating to their homogeneity and the degree of solubility of the drug substance, both of which may have an important bearing on biological effects.

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Stability Matters in Drug Discovery

In this article I have discussed several types of stability assay important in Drug Discovery including stability in simple buffer systems at various pH values, two approaches to photostability and its relationship with phototoxicity, glutathione reactivity and chiral stability assays.

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